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Sains Malaysiana 54(1)(2025): 151-164
http://doi.org/10.17576/jsm-2025-5401-12
Biological
Control of Aspergillus flavus with Pseudozyma hubeiensisYeast from Nutmeg (Myristica fragrans Houtt.)
(Kawalan Biologi Aspergillus flavus dengan Yis Pseudozyma hubeiensis daripada Buah Pala (Myristica fragrans Houtt.))
DWI N
SUSILOWATI1, CAHYANI HASNA DESWANTI2, NANI RADIASTUTI2,
SRI RAHAJOENINGSIH1, YADI SURYADI1,*,
SUPRIADI3, NURUL HIDAYAH3, SRI WIDAWATI4 &
NINIK SETYOWATI5
1Research Centre for Horticulture-National
Research and Innovation Agency, Jl. Raya Jakarta-Bogor km 46, Cibinong Bogor
16915, West Java Indonesia
Received: 11
March 2024/Accepted: 28 October 2024
Abstract
Yeasts
are potential biocontrol agents for Aspergillus flavus, an
aflatoxin-producing fungus that is present in various agricultural products,
including nutmeg. This study aimed to obtain yeast isolates from nutmeg (seeds,
pulps, and leaves), characterise them, and identify their antagonistic effects
on A. flavus. The antagonistic activities toward A. flavus were
determined by a dual-culture method. Moreover, the possible mechanism
responsible for these antagonistic effects was also analysed. The results
showed that 51 yeast isolates were successfully isolated from nutmeg. The
inhibition percentages of 47.25 ± 1.66% (isolate DP 1341a) and 55.98 ± 1.31%
(isolate DP 1342) were statistically significant (p < 0.05). The
antagonistic mechanisms of the DP 1341a isolate were associated with the
production of volatile organic compounds (32.79 ± 1.01%), a chitinolytic index
(2.51 ± 0.55), and hyperparasitism but not toxin
activity. Moreover, the DP 1342 isolate produced volatile organic compounds
(54.33 ± 3.13%), exhibited toxin activity (2.74 ± 0.22) and exhibited hyperparasitism but did not exhibit chitinase activity.
Molecular identification showed that the two yeast isolates (DP 1341a and DP
1342) were identified as Pseudozyma hubeiensiswith sequence similarity > 99%.
Therefore, the selected yeast isolates, P. hubeiensis DP 1341a and DP 1342, could be further developed as biological control agents
for A. flavus. This finding will also be useful for improving biological
control agents as an eco-friendly and economically viable disease management
strategy.
Keywords:
Antagonist: Aspergillus flavus; Myristica fragrans; Pseudozyma hubeiensis; yeasts
Abstrak
Yis merupakan agen kawalan bio yang berpotensi untuk Aspergillus flavus, iaitu sejenis kulat penghasil aflatoksin, yang hadir dalam pelbagai produk pertanian termasuk buah pala.
Kajian ini bertujuan untuk memperoleh pencilan yis daripada buah pala (biji, pulpa dan daun), dan kemudian mencirikan serta mengenal pasti aktiviti antagonis terhadap A. flavus. Aktiviti antagonis terhadap A. flavus ditentukan dengan kaedah dwi-kultur. Selain itu, mekanisme yang mungkin bertanggungjawab bagi kesan antagonis juga dianalisis. Hasil kajian menunjukkan 51 pencilan yis berjaya dipencilkan daripada buah pala. Peratusan perencatan sebanyak 47.25 ±
1.66% (pencilan DP 1341a) dan 55.98 ± 1.31% (pencilan DP 1342) adalah signifikan secara statistik (p < 0.05). Mekanisme antagonis pencilan DP 1341a dikaitkan dengan pengeluaran sebatian organik meruap (32.79 ± 1.01%), indeks kitinolitik (2.51 ± 0.55), hiperparasitisme, tetapi tidak menghasilkan aktiviti toksin. Manakala, pencilan DP 1342 menghasilkan sebatian organik meruap (54.33 ± 3.13%), menunjukkan aktiviti toksin (2.74 ± 0.22) dan hiperparasit, tetapi tidak menunjukkan aktiviti kitinase. Pencirian molekul menunjukkan bahawa kedua-dua pencilan yis tersebut (DP 1341a dan DP 1342) telah dikenal pasti sebagai Pseudozyma hubeiensis dengan persamaan jujukan > 99%. Oleh itu, pencilan yis P. hubeiensis DP 1341a dan DP 1342 yang terpilih boleh dibangunkan sebagai agen kawalan biologi bagi A. flavus. Penemuan ini juga berguna untuk penambahbaikan agen kawalan biologi sebagai strategi pengurusan penyakit yang mesra alam dan berdaya maju dari segi ekonomi.
Kata kunci: Antagonis; Aspergillus
flavus; Myristica fragrans; Pseudozyma hubeiensis; yis
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